IN VITRO MORPHOGENIC PERFORMANCE OF GINGER (ZINGIBER OFFICINALE) AS INFLUENCED BY MEDIA VARIATIONS

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IN VITRO MORPHOGENIC PERFORMANCE OF GINGER (ZINGIBER OFFICINALE) AS INFLUENCED BY MEDIA VARIATIONS

ABSTRACT

Ginger (Zingiber officinale Rose) is a perennial herb that grows from underground

rhizomes. The demand for fresh and dry ginger and its essential oil in the world market

is high. Ginger is propagated vegetatively using underground rhizomes. Most farmers

use planting materials saved from previous harvest. These materials could have been

sold for cash. Contingent on this, many farmers are reluctant to use healthy succulent

rhizomes for planting. These are rather sold thereby resulting in acute shortage of

planting materials.

Tissue culture technique can be applied to mass-produce seedlings

for distribution to ginger farmers. This however, is not cost effective now due to lack of

the necessary materials such as agar required for tissue culture. It is on the basis of

these considerations that the present research was set up to develop a locally available

and cheap protocol that is reproducible in ginger plant tissue culture work with the

following objectives:

To determine the best pre-initiation treatment for in-vitro ginger

multiplication; To determine the effect of plant growth hormones – auxin and cytokinin

on in-vitro propagation of ginger at the initiation stage using agar as a gelling agent; To

compare agar gelled medium with cassava gelled medium in in-vitro propagation of

ginger at the initiation stage. The research was carried out in the tissue culture research

laboratory of the National Root Crop Research Institute (NRCRI), Umudike Station,

Umuahia, Abia State. The explants were collected from ginger germplasm of the

Institute. The result shows that Sodium Hypochlorite (NaOCl) as 30% bleach and

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