IN VITRO ANTIMICROBIAL ACTIVITY OF ACACIA NILOTICA PODS AGAINST SELECTED REFERENCE BACTERIAL STRAINS

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IN VITRO ANTIMICROBIAL ACTIVITY OF ACACIA NILOTICA PODS AGAINST SELECTED REFERENCE BACTERIAL STRAINS

Abstract

This was a descriptive experimental study conducted in Sudan University of Science and Technology (College of Medical Laboratory Sciences). The aim of this study was to determine the antimicrobial activity of different concentrations of Acacia nilotica pods extracts against selected reference bacterial strains using ethanol, chloroform, and aqueous extracts by agar diffusion method. Six reference bacterial strains were tested [Escherichia coli (ATCC 25922), Klebsiella pnemoniae (ATCC 27736), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 49461), and Bacillus cereus (ATCC 10876)] and the antimicrobial activity was determined against Acacia nilotica extracts.The ethanolic extract of Acacia nilotica was active against all reference strains. The aqueous extract of Acacia nilotica was active against Staphylococcus aureus and Bacillus cereus with moderate activity against Escherichia coli, Klebsiella pnemoniae, Pseudomonas aeruginosa, and Staphylococcus epidermidis. All reference strains were resistant to the chloroform extract of Acacia nilotica. The minimum inhibitory concentration (MICs) for the tested plant extracts showed that the ethanolic extract was more active than aqueous extract with MICs 6.25 g/ml for 5(6) reference bacterial strains, while the aqueous extract gave MICs ≥ 25 g/ml. Further studies are required to confirm these results.

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